Carotenoid levels in human lymphocytes, measured by Raman microspectroscopy
نویسندگان
چکیده
منابع مشابه
Intracellular carotenoid levels measured by Raman microspectroscopy: comparison of lymphocytes from lung cancer patients and healthy individuals.
Most studies concerning a possible protective role of carotenoids against cancer focus on serum carotenoid levels. We have used Raman microspectroscopy to study the intracellular amounts of carotenoids in lymphocytes of lung cancer patients and of healthy individuals. Our results indicate a significant decrease of carotenoids in lung carcinoma patients compared with healthy individuals, particu...
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Raman spectra were obtained from fresh, fixed and sliced rabbit lenses and from human lens slices. For all lenses and lens slices the ratio R, defined as the Raman intensity at 3390 cm-1 divided by the Raman intensity at 2935 cm-1, was measured at different locations along the visual and equatorial axis. The ratios R were transformed to absolute water mass percentages by measuring solutions wit...
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The Raman microspectroscopic method was used to determine the local water and protein content in human lenses. In 18 lenses of varying age position-defined water/protein content measurements were carried out along the visual and the equatorial axis. A main characteristic of the human lens is its constant and relatively low protein content. In addition this constant nuclear value is reached with...
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In previous studies (Huizinga et al., 1989; Bot et al., 1989) it was demonstrated that the results of water content measurements of human and rabbit lenses estimated by Raman microspectroscopy were comparable to those using differential scanning calorimetry, nuclear magnetic resonance spectroscopy, dynamic light scattering and the dry-weight-frozensection technique. It was emphasized that Raman...
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The conformation of low-salt LiDNA in fibrous form has been studied by Raman microspectroscopy. The recorded spectrum of a fibre soaked in ethanol is very similar to that of a fibre at low relative humidity. From x-ray experiments it is known that in the latter case the DNA adopts the C conformation. Therefore, it can be concluded that this is also the case in a low-salt LiDNA fibre soaked in e...
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ژورنال
عنوان ژورنال: Pure and Applied Chemistry
سال: 1997
ISSN: 1365-3075,0033-4545
DOI: 10.1351/pac199769102131